Fig. 8: Wild-type DKC1 reverses SENP3-mediated anti-metastasis effects and is associated with poor prognosis in PDAC patients.
From: SUMO specific peptidase 3 halts pancreatic ductal adenocarcinoma metastasis via deSUMOylating DKC1
A–D The effects of wild-type DKC1 and SUMOylation-resistant DKC1 (3KR) overexpression on PDAC cell lines with overexpressing SENP3 were determined by transwell assay as well as in vivo migration imaging. Wild-type DKC1 overexpression suppressed the anti-metastasis effects of SENP3, but mutated DKC1 with 3 K-R mutant sites could not. unpaired T test. E, F DKC1 expression was detected in the same PDAC tissue microarray panel shown in Fig. 1 and the correlation between SENP3 and DKC1 at protein level was analyzed. Representative images were shown in E, linear-regression analysis based on IHC scores was presented in F. G–I Overall survival based on DKC1 expression among all patients or in stratified groups according to SENP3 expression. log-rank test. ns, no significance, **p < 0.01, ***p < 0.001, ****p < 0.0001.
