Fig. 1: Hnrnpk is expressed in developing limb buds and is essential for proper limb bud formation. | Cell Death & Differentiation

Fig. 1: Hnrnpk is expressed in developing limb buds and is essential for proper limb bud formation.

From: Hnrnpk is essential for embryonic limb bud development as a transcription activator and a collaborator of insulator protein Ctcf

Fig. 1

A Representative WISH images showed Hnrnpk (left panel) and Sox9 (middle panel) expression at E10.5 (top), E11.5 (middle), and E12.5 (bottom) limb buds. The Hnrnpk-diagram (right panel) presented the range of Hnrnpk signals. The black arrows indicated stronger signals detected in the periphery and cartilage primordia region of the limb buds. Scale bar: 1 mm. B Construction protocol of floxed-Hnrnpk mice. Exons 4–7 of the Hnrnpk gene was flanked by loxP sites. The horizontal lines indicated chromatin, vertical lines indicated the exons of Hnrnpk, yellow line represented the exon of adjacent gene Rmi1, and triangles indicated loxP sites inserted. C The efficient knockout of Hnrnpk in limb buds was confirmed by WB using proteins from E11.5 WT and CKO limb buds. β-Actin was used as the loading control. D Representative images of general observation (left panel) and skeletal preparation (right panel) of E18.5 WT and CKO embryos. The black arrow indicated the hematoma on the E18.5 CKO head. Scale bar: 1 mm. E Representative images of general observation of E12.5 WT and CKO embryos. The dotted boxes showed regions of mesenchyme condensation. Scale bar: 1 mm. F Representative Lyso tracker images of E11.5 WT and CKO limb buds. The white boxes indicated the magnification of the forelimb. Scale bar: 1 mm.

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