Fig. 5: STING directly binds to YY1 and prevents YY1 nuclear translocation to increase LCN2 transcription.

A p-STING-bound protein YY1 in astrocytes is identified by LC-MS/MS. B The Venn diagram presents the overlap of the predicted transcription factors of LCN2 between the PROMO, UCSC, and Animal TFDB datasets. C Binding of YY1 to LCN2 promoter was analyzed using ChIP-qPCR in HEK293 cells (Five independent experiments). IgG, immunoglobulin G. D Luciferase reporter activity of LCN2 in HEK293 cells transfected with YY1-expressing plasmid or vector (Five independent experiments). E Representative immunoblots of LCN2 expression and YY1 in astrocytes transfected with YY1 siRNA (si-YY1) or control siRNA (si-control) (Four independent experiments). F Immunoprecipitation and immunoblot analysis of the interaction of p-STING with YY1 in astrocytes treated with MPP⁺ using anti-p-STING and anti-YY1 antibodies (Three independent experiments). G Immunoprecipitation and immunoblot analysis of the interaction of p-STING with YY1 in astrocytes cultured for 7 days or 40 days (Three independent experiments). H Representative images showing the localization of p-STING (red) and YY1 (green) in astrocytes. I Representative Immunoblot of YY1 expression in the nucleus and the cytoplasm from astrocytes treated with MPP⁺. J Representative Immunoblot of YY1 expression in the nucleus and the cytoplasm from astrocytes cultured for 7 days or 40 days. K Representative images of immunofluorescence staining on YY1 (green) and DAPI (blue) in astrocytes transfected with cGAS/STING siRNA and then treated with MPP⁺. The data shown are the mean ± SEM. Unpaired t test was used (C, D).