Fig. 6: LCN2 is required for cGAS-STING-mediated astrocyte senescence and neurodegeneration in MPTP treated mice. | Cell Death & Differentiation

Fig. 6: LCN2 is required for cGAS-STING-mediated astrocyte senescence and neurodegeneration in MPTP treated mice.

From: The cGAS-STING-YY1 axis accelerates progression of neurodegeneration in a mouse model of Parkinson’s disease via LCN2-dependent astrocyte senescence

Fig. 6

A Representative pictures of MAP2 (green) immunostaining. DAPI stains nucleus (blue). Quantification of relative the number of neurons (B, four independent experiments) and mean total neuritis length (C, four independent experiments). D The time taken to descend a pole (Time-total) was recorded in pole test (n = 14 animals for each group). E Time on the rod was measured in the rotarod test (n = 10 animals for each group). F, G Movement distance within 5 min was recorded in open field test (n = 13 animals for each group). H Microphotographs of TH-positive neurons in the SNpc. I Stereological counts of TH-positive neurons in the SNpc (n = 6 animals for each group). Representative immunoblots (J) and quantification of TH (K) and p16 expression (L) in the SNpc (n = 5 animals for each group). qPCR measurement of IL-1α (M), IL-1β (N), IL-6 (O), and MMP9 (P) mRNA expression in the SNpc (n = 6 animals for each group). Q Representative double-immunostaining for lamin B1 (red) and EGFP (green) in astrocytes in the SNpc. R Quantification of lamin B1 immunofluorescence intensity in GFAP+ astrocytes in the SNpc (n = 6 animals for each group). DAPI stains nucleus (blue).The data shown are the mean ± SEM. Two-way ANOVA with Tukey’s post-hoc tests were used.

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