Fig. 3: Pinch loss significantly stimulates inflammatory response related genes in monocytes.

A Visualization of bone marrow hematopoietic cells in 6-wk-old male control and dKO mice in UMAP using scRNA-seq. B Cell proportion of each cell type in 6-wk-old male control and dKO mice using scRNA-seq. C Cell proportion of monocyte lineage cells in control group and dKO group. D Expression levels of immune related genes in GMPs, monocyte progenitors (MoPro), and Monocytes (Mo) in 6-wk-old male control and dKO mice. E Expression level of Cxcr4 in neutrophil progenitors, immature neutrophils, mature neutrophils, monocyte progenitors, and monocytes. F A diagram of co-culture system. BMSCs are seeded in the top wells, and RAW264.7 cells or primary bone marrow monocytes were seeded in the bottom wells, with pretreatment with or without LPS. TNF-α (G) and IL-1β (H) concentrations in the culture medium of RAW264.7 cells after co-cultured with BMSC or BMSC^dKO followed by LPS challenge (n = 6). Data are mean ± SD of 3 independent experiments. TNF-α (I) and IL-1β (J) concentrations in the culture medium of primary bone marrow monocytes after co-cultured with BMSC or BMSC^dKO followed by LPS challenge (n = 6). Data are mean ± SD of 3 independent experiments. K Serum TNF-α and IL-1β concentrations in 6-wk-old male control and dKO mice (n = 6). Data are mean ± SD of 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.