Fig. 4: cGAS interacts with Ku80 to promote USP7-mediated stabilization of DNA-PKcs.
From: The cGAS-Ku80 complex regulates the balance between two end joining subpathways
A Co-IP analysis of the interaction between cGAS and c-NHEJ factors in HEK293T cells. Cells were transfected with cGAS-HA and lysed for the co-IP assay. B In vitro co-IP analysis of the interaction between cGAS and Ku80 or Ku70. The purified recombinant proteins were incubated, and a co-IP assay with an antibody against GST was performed, followed by Western blotting analysis. C Co-IP analysis of the interaction between endogenous cGAS and Ku80 in HCA2-hTERT cells exposed to X-ray irradiation (8 Gy, 0.5 h). D Co-IP analysis of the interaction between cGAS-HA and Ku80 following subcellular fractionation. E Immunofluorescence images of cGAS-GFP and endogenous Ku80 upon laser microirradiation in U2OS cells. Scale bar, 5 μm. F Co-IP analysis of the endogenous USP7-DNA-PKcs interaction in control or Ku80-depleted cells with or without cGAS overexpression. G Analysis of DNA-PKcs expression in Ku80-depleted cells with or without cGAS overexpression. H Co-IP analysis of the interaction between endogenous USP7 and Ku80. I Co-IP analysis of the interaction between endogenous USP7 and Ku80 with or without cGAS overexpression.
