Fig. 1: MLKL-mediated necroptosis causes skin inflammation in FADDE-KO mice.
A Representative photographs of FADDE-KO (n = 7) mouse with unaffected littermate (n = 4) at P7 and of FADDE-KO Mlkl−/− (n = 8) mouse with WT littermate (n = 8) at 48–50 weeks. B Graph depicting lesion onset of mice with indicated genotypes. C Representative images from skin sections of FADDE-KO mouse, FADDE-KO Mlkl−/− mouse and littermates stained with H&E (Scale bars = 100 µM, n = 7 for FADDE-KO, n = 4 for control, n = 5 for FADDE-KO Mlkl−/−, n = 4 for control) or immunostained with K10, K14 and K6 (Scale bars = 50 µM, n = 4 for FADDE-KO, n = 3 for control, n = 5 for FADDE-KO Mlkl−/−, n = 4 for control). D Graph depicting epidermal thickness of mice with indicated genotypes. E Graphs depicting relative mRNA expression of the indicated cytokines and ISGs in RNA from whole-skin tissue of mice at P7 of the indicated genotypes measured by qRT-PCR. Each dot represents one mouse. Mean ± SEM are shown. F Representative images from skin sections of Casp8E-KO MlklAA/WT, Casp8E-KO MlklAA/AA and healthy control stained with H&E (Scale bar 100 µM, n = 4 for Casp8E-KO MlklAA/WT, n = 4 for Casp8E-KO MlklAA/AA, n = 4 for control). G Graphs depicting relative mRNA expression of the indicated cytokines and ISGs in RNA from whole-skin tissue of mice at P7-10 of the indicated genotypes measured by qRT-PCR). Statistical significance was determined using Kruskal–Wallis test (one-sided) in (E) and (G).
