Fig. 2: Distance between suppressive immune cells and LMO7⁺ cells decreased in PDAC.

a The schematic representation illustrating the experimental design and analytical methods employed in this study. b The mIHC panel for PDAC tissues, incorporating LMO7 and immunological markers. c Representative images depicting staining with antibodies against the indicated marker. d Proportion of CD3⁺ lymphocytes co-expressing FOXP3. e Proportion of CD3⁺ CD8⁺ lymphocytes co-expressing GranzymeB. f Proportion of CD3⁺ CD8⁺ lymphocytes without co-expressing GranzymeB. g CD3⁺ T cells percentage of all nucleated cells. h Proportion of CD3⁺ lymphocytes co-expressing CD8. i Halo spatial analysis plot illustrating LMO7⁺ cells (blue), immune cells within 100 μm (orange), and those >100 μm away (red) from an LMO7⁺ cell. j Average distance to LMO7⁺ cells for each CD3⁺ FOXP3⁺ T cell. k Average distance to LMO7⁺ cells for each CD3⁺ CD8⁺ GranzymeB⁺ T cells. l Average distance to LMO7⁺ cells for each CD3⁺ CD8⁺ GranzymeB^- T cells. m Proportion of unique LMO7 cells. Data are presented as mean ± SEM. The p-value was determined using unpaired two-sided Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.