Fig. 5: Specific lineage tracing reveals hepatocyte reprogramming in mice ICC. | Cell Death & Differentiation

Fig. 5: Specific lineage tracing reveals hepatocyte reprogramming in mice ICC.

From: Hepatic factor MANF drives hepatocytes reprogramming by detaining cytosolic CK19 in intrahepatic cholangiocarcinoma

Fig. 5

A, B Identification of the specificity of tdTomato (red) in mature hepatocytes after AAV8-TBG-Cre injection. Hepatocytes were labeled with anti-HNF4α antibody (green). The nonparenchymal cells were labeled with anti-CK19 (green, BECs marker), α-SMA (green, hepatic stellate cells marker), and F4/80 (green, macrophages marker) antibodies, respectively. C Identification of high specificity of AAV8-TBG-Cre for HNF4α+ hepatocytes. D Identification of double-positive hepatocytes in the liver tissues of SBT-induced ICC mice with tdTomato (red) and anti-CK19 (green) antibody. E Identification of HNF4α+CK19+ cells in TAA-induced ICC mice with anti-HNF4α (green) and anti-CK19 (red) antibodies, respectively. F Multiple lineages reprogramming of hepatocytes in SBT-induced mice ICC double labeled by tdTomato and anti-α-SMA (green), anti-F4/80 (green), and anti-CD133 (green), respectively. G The proportion of reprogramming from hepatocytes to myofibroblasts in mice ICC induced by SBT was calculated by anti-HA (red) and anti-α-SMA (green). The nuclei were stained with DAPI (blue).

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