Fig. 3: Lumican co-localized with tubulin and regulated tubulin expression.
From: Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
a The total cell lysates of H460 and H460LD cells underwent western blot analysis, and the levels of tubulin and lumican were detected. GAPDH was a loading control for the cytosol fraction. b Immunoprecipitation detection of lumican and tubulin. Cell lysates were immunoprecipitated with anti-β-tubulin or lumican antibodies. The immunoprecipitates underwent western blot analysis with indicated antibodies. c Fluorescence staining of lumican (green) and tubulin (red), in LD cells and control cells. The indicated cells were fixed with paraformaldehyde and co-incubated with anti-tubulin to detect the microtubule expression. Cell nuclei were visualized by DAPI staining (blue). N = 3. Significantly different from control (*p < 0.05).
