Fig. 8: Knockout of lumican by gRNAs/Cas9 induced morphological change and cell death.
From: Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin
A549 and H460 cells were transfected with lumican CRISPR/Cas9 KO plasmid (gRNAs/Cas9) (0, 0.25, 0.5, and 1 μg/ml) for 48 h. a The cell viability was investigated by the colorimetric MTS assay. b The LDH activity in the conditioned media was measured by the CytoTox 96 assay. c The cell morphology were shown in higher magnification (10x40) after transfection of Crispr/Casp9 Lum for 48 h. d The cell lysates were subjected to western blotting with indicated antibodies. The expressions of lumican, α-tubulin, and full length PARP were determined by densitometry of the blots and expressed in relative arbitrary units. e The active caspase-3 was detected by Caspase 3/7-GLO assay. Caspase 3/7 activity of gRNAs/Cas9-untreated control group of each cell line was set as 1. Activities of transfected cells were compared to control group. The data were expressed as the mean ± SD of three independent experiments. Significantly different from control (*p < 0.05, **p < 0.01,***p<0.001).
