Fig. 6: miR-125b inhibits EVA1A induced autophagy in oxaliplatin resistance. | Cell Death & Disease

Fig. 6: miR-125b inhibits EVA1A induced autophagy in oxaliplatin resistance.

From: MicroRNA-125b reverses oxaliplatin resistance in hepatocellular carcinoma by negatively regulating EVA1A mediated autophagy

Fig. 6

a, b Representative images of immunofluorescence staining for mRFP-GFP-LC3 in SK-Hep1 and HepG2 cells. Representative profiles of autophagosomes (RFP + GFP + dots) and autolysosomes (RFP + GFP- dots) per cell section assessed by confocal microscopy are shown and were quantified. c, d Western blotting analysis of autophagy-related proteins and quantified levels of proteins. e, f The autophagic vacuoles (autophagosomes) were detected by transmission electron microscopy (TEM). The representative TEM images are shown and the typical autophagosomes are marked with red arrows. The number of autophagosomes per cell was calculated by counting the number of double-membrane organelles in 10 cells. Data are expressed as means ± SEM (n = 3). *P < 0.05 compared to pCDH, #P < 0.05 compared to EVA1A

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