Cell Death & Disease

Table 1 Sources and dilutions of primary antibodies

From: Rapamycin and fasting sustain autophagy response activated by ischemia/reperfusion injury and promote retinal ganglion cell survival

Target	Supplier and catalog no.	Method and dilution
LC3	MBL, PM036	WB, 1:2000
p62	Sigma, P0067	WB, 1:4000
p62	Santa Cruz, sc-25575	IFC, 1:50
Beclin1	Cell Signaling, 3495	WB, 1:1000
4EBP1	Cell Signaling, 9644	WB, 1:1000
p-4EBP1 (Thr37/46)	Cell Signaling, 2855	WB, 1:1000
ULK1	Cell Signaling, 8054	WB, 1:1000
p-ULK1 (Ser757)	Cell Signaling, 6888	WB, 1:1000
mTOR	Cell Signaling, 2972	WB, 1:1000
p-mTOR (Ser2448)	Cell Signaling, 2971	WB, 1:1000
AMPK	Cell Signaling, 2603	WB, 1:1000
pAMPK (Thr172)	Cell Signaling, 2535	WB, 1:1000
ATG4B	Cell Signaling, 5299	WB, 1:1000
ATG7	Cell Signaling, 2631	WB, 1:1000
ATG12	Cell Signaling, 4180	WB, 1:1000
β-actin	Sigma, A5441	WB, 1:30000
GAPDH	Applied Biosystem, AM4300	WB, 1:30000
LAMP-2	Pierce Biotechnology, PA1-655	IFC, 1:50
TUJ1	BioLegend, MMS-435P	IFC, 1:500

WB western blotting, IFC immunofluorescence

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