Fig. 3: VEGFA is required for angiogenesis mediated by SIRT2. | Cell Death & Disease

Fig. 3: VEGFA is required for angiogenesis mediated by SIRT2.

From: Inhibition of SIRT2 limits tumour angiogenesis via inactivation of the STAT3/VEGFA signalling pathway

Fig. 3

a The mRNA levels of the indicated genes in ShNC SW48 cells and ShSIRT2 SW48 cells were examined by qPCR assay. Each bar represents the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001. b Western blot assay showing the VEGFA protein expression in the ShNC and ShSIRT2 CRC cell lines. c, d VEGFA production was examined by ELISA in the indicated culture supernatants. Each bar represents the mean ± SD of three independent experiments. **P < 0.01. e, f Wound healing assay showing the migratory ability of HUVECs treated with conditional medium (CM) from ShNC SW480 cells and ShSIRT2 SW480 cells or treated with CM added with VEGFA (100 ng/ml). The migration distance is shown in the bar graph. Each bar represents the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001. g, h Effect of CM from ShNC SW480 cells and ShSIRT2 SW480 cells or CM added with VEGFA (100 ng/ml) on tube formation in HUVECs. The number of tubes counted is shown in the bar graph. Each bar represents the mean ± SD of three independent experiments. *P < 0.05

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