Fig. 4: SHROOM2 is required for epithelial morphology maintenance. | Cell Death & Disease

Fig. 4: SHROOM2 is required for epithelial morphology maintenance.

From: SHROOM2 inhibits tumor metastasis through RhoA–ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma

Fig. 4

a Morphology of control and SHROOM2 knockdown HONE1 cells observed by bright field microscopy. b (top) Colonies formed by control and SHROOM2 knockdown HONE1 cells on conventional tissue culture plates. (middle) Representative colony images as classified with Type I, II and III. (bottom) Quantification of different types of colonies formed by control and SHROOM2 knockdown cells. c Morphological features of control and SHROOM2-depleted HONE1 cells in soft agar culture. Scale bar: 50 μm. d Quantification of spheres formed in c with irregular shapes (left) and total number of the spheres (right). e (top) Morphology and (bottom) quantification of spheres with irregular shape of control and SHROOM2-depleted HONE1 cells grown in Matrigel. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed unpaired t-test

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