Fig. 5: Knockdown of miR-210-3p suppresses endometriosis progression in vivo. | Cell Death & Disease

Fig. 5: Knockdown of miR-210-3p suppresses endometriosis progression in vivo.

From: MiR-210-3p protects endometriotic cells from oxidative stress-induced cell cycle arrest by targeting BARD1

Fig. 5

a Schematic diagram of mouse model of endometriosis. Mice received intraperitoneal (IP) transplantation of uterine tissue from donor mice and were treated with miR-210-3p inhibitor (In-210) or a negative control (NC) using an in vivo-jet PEI delivery agent via intraperitoneal injection every other day. All mice were supplied with 200 mg/kg 17β-estradiol (E2) every other day. After 4 weeks, endometriotic cysts were excised to harvest the extent of endometriosis. b Upper panel showing representative visible lesions within the peritoneal cavity of an NC mouse and In-210 mouse. Lower panel showing scatter plot of lesion volumes from NC and In-210 mice (n = 8). *P < 0.05 vs. NC, Student's t-test. c Scatter plot showing expression of BARD1 and BRCA1 in stromal cells and glandular cells from NC or In-210 treated lesions by H-score (y-axis) (n = 8). *P < 0.05 vs. NC, paired Student t-test. d Immunohistochemistry photomicrographs of BARD1 and BRCA1 expression in NC- or In-210 treated lesions. Scale bar = 100 μm for ×100images. Scale bar = 50 μm for ×400 images

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