Fig. 2: MiR-184 promotes the apoptosis and inhibits the proliferation of trophoblast cells.

a HTR8 cells were infected with negative control lentivirus and miR-184 overexpression lentivirus for 72 h, and GFP-positive cells were sorted out. Flow cytometry was used to detect the infection efficiency. b We also used RT-PCR to detect the mRNA expression of miR-184 in NC and miR-184 cells. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. ****P < 0.0001. Data represent mean ± SEM. c CCK8 was used to detect cell proliferation of NC and miR-184 cells, which indicated that miR-184 could markedly inhibit cell proliferation. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. **P < 0.01. Data represent mean ± SEM. d After culturing NC and miR-184 cells for 48 h, the Annexin V staining result showed that miR-184 could increase cell apoptosis significantly. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. *P < 0.05. Data represent mean ± SEM. e We examined the invasiveness of NC and miR-184 cells via transwell assay, finding that miR-184 had little effect on the invasiveness of trophoblast cells. Scale bar: 100 μm. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. Data represent mean ± SEM. These experiments were repeated three times. (miR-184: HTR8 cells infected with miR-184 overexpression lentivirus; NC: HTR8 cells infected with negative control lentivirus)