Fig. 4: WIG1 (ZMAT3) is a functional target gene of miR-184.

a We detected the protein levels of AGO2, LASP1, TMED2, FOXP3, Pdx1 FoxO3a, MAFG, iASPP, and WIG1 in NC and miR-184 overexpression cells via western blot analysis, and found that only the expression of AGO2, LASP1, MAFG, and WIG1 was decreased in miR-184 overexpression cells. b Luciferase reporter vectors containing the wild-type or mutant 3’UTRs of AGO2, LASP1, MAFG, and WIG1 were cotransfected with miR-184 plasmid into HEK293T cells for 48 h, and luciferase reporter assays were performed to define the target genes. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. *P < 0.05, **P < 0.01. Data represent mean ± SEM. c After transfection with siRNAs for 48 h, expression of AGO2, LASP1, MAFG, WIG1 and the expression of Fas in HTR8 cells was detected via western blot analysis. d Flow cytometry was used to confirm the result obtained from western blot analysis. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. **P < 0.01. Data represent mean ± SEM. These experiments were repeated three times