Fig. 4: Brefeldin A reduced SQ1 and SQ2 induced translocation of LC3 to the Golgi.
From: Squaramide-based synthetic chloride transporters activate TFEB but block autophagic flux
a, b Representative images of U2OS GFP-GALT RFP-LC3 cells treated with the compounds for 6 h (oleate 500 μM, SQ1, SQ2 at 10 μM) without (a) or after pretreatment with brefeldinA (10 μg/ml) for 1 h (b). c–e Statistical analysis of RFP-LC3 puncta counts per cell, GFP-GALT marked Golgi surface per cell and the localization of LC3 and GALT (***p < 0.0001, compared to untreated control cells, Co)
