Fig. 5: Contribution of TFEB and ATG5 in LC3 relocation induced by the SQs.
From: Squaramide-based synthetic chloride transporters activate TFEB but block autophagic flux
a, b Representative images of U2OS cells stably expressing GFP-TFEB fusion protein, treated with torin 300 nM, SQ1, SQ2, and SQ3 at 10 μM during 6 h. Data are expressed as means ± SEM of at least three independent experiments and demonstrate the average ratio between GFP-TFEB florescence intensity in the nucleus vs. cytoplasm. (*p < 0.05, **p < 0.01, ***p < 0.0001, compared to untreated cells, Co). c, d Representative images and statistics of U2OS GFP-LC3 cells, and the ATG5 KO and TFEB KO counterparts after 6 h of treatment with the mentioned compounds. Bars depicts the absolute number of GFP-LC3+ dots per cell. Data are expressed as means ± SEM of at least three independent experiments (*p < 0.05, ***p < 0.0001, compared to untreated cells, Co)
