Fig. 3: TP73-AS1 promotes self-renewal in G7 but not in G26 gCSC. | Cell Death & Disease

Fig. 3: TP73-AS1 promotes self-renewal in G7 but not in G26 gCSC.

From: The lncRNA TP73-AS1 is linked to aggressiveness in glioblastoma and promotes temozolomide resistance in glioblastoma cancer stem cells

Fig. 3

a gCSC cells expressing a doxycycline-inducible dCAS9-KREB fusion protein and the indicated gRNAs targeting TP73-AS1 promoter region were generated. dCAS9-KREB expression was induced for 10 days, after which the expression of the indicated stemness and differentiation markers was determined using immunofluorescence. Representative images are shown. Bar = 100 μM. b LDA assay measures the self-renewal capacity. dCAS9-KREB expression was induced for 10 days, after which the cells were plated in 96-well plates in the concentration of (1, 5, 10, and 20 cells per well). Spheres were counted manually 21 days after the induction. The estimate depicts the confidence interval for one per stem cell frequency. Data were analyzed using http://bioinf.wehi.edu.au/software/elda/

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