Fig. 4: TP73-AS1 promotes gCSC resistance to TMZ.

a Representative images of TP73-AS1 depleted and control gCSC cells treated with TMZ (500 µM) for 7 days. Images were recorded using a light microscope. b TP73-AS1 depleted and control gCSC models were treated with TMZ (500 µM) for 7 days, after which the viability was determined using Annexin V/PI staining and flow cytometry. Representative images are shown. Bar = 50 μM; *p < 0.05; n = 3; average ± SD. c TP73-AS1 depleted and control gCSC were treated with the indicated dose of TMZ for 7 days, after which viability was measured using crystal violet staining and a plate reader. Values represent the relative average OD; n ≥ 3; *p < 0.05 Student’s t test