Fig. 3: GSNOR localization in skeletal muscle.
From: nNOS/GSNOR interaction contributes to skeletal muscle differentiation and homeostasis
a Representative fluorescence microscopy images of tibialis anterior sections from wild-type (WT) mice stained with anti-GSNOR and anti-collagen III (Col III) antibodies. b Immunoprecipitation (IP) of tibialis anterior homogenates from 2-months-old WT and Gsnor−/− (KO) mice performed with an anti-nNOS antibody and revealed by western blot (IB) for GSNOR and nNOS. Gsnor−/− (KO) muscles were selected as control. c Immunoprecipitation (IP), as described in b, of lysates from C2C12 myoblasts induced to differentiate upon 4 days of serum deprivation. d Representative fluorescence microscopy images of human specimens of rectus abdominis stained with anti-GSNOR and anti-nNOS antibodies, or e with anti-GSNOR and DAPI to highlight the nuclear/perinuclear localization
