Fig. 3: Activation of NLRP3-ASC is involved in macrophage pyroptosis induced by propofol. | Cell Death & Disease

Fig. 3: Activation of NLRP3-ASC is involved in macrophage pyroptosis induced by propofol.

From: Propofol directly induces caspase-1-dependent macrophage pyroptosis through the NLRP3-ASC inflammasome

Fig. 3

a BMDMs were exposed to 0, 12, 60, 300 and 600 μM propofol. Following 3 h of propofol exposure, immunoblots were performed to detect pyroptosis-related proteins in cell extracts. Quantitative accumulated western blot data for inflammasomes and ASC are shown. b Western blot analysis was used to determine the expression of inflammasomes and ASC. BMDMs were treated with propofol (300 μM) or vehicle for the indicated time periods. GAPDH was an internal control. Quantitative accumulated western blot data for inflammasomes and ASC are shown. c NLRP3 knockout BMDMs induce pyroptosis after exposure to propofol for 3 h. Supernatant LDH activity was assessed, and OD values at 490 nm are presented in the histogram. d BMDMs were exposed to 0, 12, 60, 300 and 600 μM propofol. Following 3 h of propofol exposure, western blot analysis of pro-caspase-1 and cleaved caspase-1 in WT and NLRP3-/- BMDMs. Band intensity of cleaved caspase-1 was quantified by ImageJ software, and the values of target proteins were analysed. e Western blot analysis of inflammasomes in NLRP3-/- BMDMs; band intensity was quantified by ImageJ software, and the values of target proteins were normalised to that of GAPDH. The results are representative of three independent experiments. The data are expressed as the mean ± SE (n = 3–6); *P < 0.05 and **P < 0.01 versus control; #P < 0.05; NS, not significant; one-way ANOVA followed by the Duncan’s test. WT = wild type; WCL = whole-cell lysates; Cell ext = cell extract; CASP1 = caspase-1

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