Fig. 6: Propofol-induced cell death is attenuated in caspase-1-/- BMDMs. | Cell Death & Disease

Fig. 6: Propofol-induced cell death is attenuated in caspase-1-/- BMDMs.

From: Propofol directly induces caspase-1-dependent macrophage pyroptosis through the NLRP3-ASC inflammasome

Fig. 6

Caspase-1-/- BMDMs were exposed to 0, 12, 60, 300 and 600 μM propofol. a Following 3 and 6 h of propofol exposure, cell viability was evaluated. b Supernatant LDH activity was measured in cultured caspase-1-/- BMDMs, and OD values at 490 nm are presented. c Immunoblots were performed to detect apoptosis-related proteins in cell extracts. d Knockout of caspase-1 aggravated propofol-induced apoptosis in BMDMs. Caspase-1-/- BMDMs were treated with propofol (300 μM) or vehicle. Following 3 h of propofol exposure, western blot analysis of apoptosis-related proteins in caspase-1-/- BMDMs was performed. e Band intensity was quantified by ImageJ software, and the values of target proteins were normalised to that of GAPDH. The results were representative of three independent experiments; *P < 0.05 and **P < 0.01 versus control; one-way ANOVA followed by the Duncan’s test. CASP1 = caspase-1; CASP3 = caspase-3; CASP7 = caspase-7; CASP9 = caspase-9; Cell ext = cell extract; WCL = whole-cell lysates

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