Fig. 3: SLAMF3^high T cells are sensitive to CD3/CD28 stimulation.

SLAMF3^high CD3⁺CD4⁺CD25⁻ T cells and SLAMF3^low CD3⁺CD4⁺CD25⁻ T cells were purified from cord blood mononuclear cells by FACS sorter, and stimulated with different concentrations of anti-CD3/anti-CD28 antibodies for 7 days in vitro (anti-CD3/anti-CD28 concentration: low 0.8 μg/ml/0.4 μg/ml, medium 2 μg/ml/1 μg/ml, and high 5 μg/ml/2.5 μg/ml). a Representative flow cytometric profiles about sorting strategy (up) and the purity of sorted SLAMF3^high or SLAMF3^low cells were shown. b Representative images of cell expansion 7 days after stimulation. Scale bars equal 200 μm. c Representative flow cytometric profiles (left) 7 days after stimulation were shown. Summarized data (right) about the ratios of live cells after expansion (n = 4 per group, mean ± SDs) based on flow cytometric analysis was shown. *p < 0.05; **p < 0.01; ***p < 0.001. (Data shown are results from a representative of three experiments)