Fig. 4: eIF3b regulated the protein expression of key genes in the cancer pathway. | Cell Death & Disease

Fig. 4: eIF3b regulated the protein expression of key genes in the cancer pathway.

From: Downregulation of eukaryotic translation initiation factor 3b inhibited proliferation and metastasis of gastric cancer

Fig. 4

a The western blot results showed that the downregulation of eIF3b inhibited the expression of E2F1, cyclin D, cyclin E, vimentin and β-catenin and increased the expression of the tumour suppressor gene P27 in SGC7901 and MGC803 cells. b The ELISA results showed that the downregulation of eIF3b inhibited the expression of IL-8 in SGC7901 cells but increased the expression of IL-8 in MGC803 cells. c The WB results showed that the pENTER-E2F1 plasmid increased the expression of E2F1. d, e The colony formation and transwell assays showed that the role of eIF3b in the proliferation and migration of SGC7901 and MGC803 cells was partially restored by E2F1. *P < 0.05, **P < 0.01, ***P < 0.001

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