Fig. 2: circPRKCI shRNA inhibits A172 glioma cell growth, survival, proliferation and migration.
From: Circular RNA PRKCI promotes glioma cell progression by inhibiting microRNA-545
A172 glioma cells were transduced with GV248 lentiviral circPRKCI shRNA (“Seq-1/Seq-2”, non-overlapping sequence) construct or scramble non-sense control shRNA (“sh-c”) construct for 24 h, stable cells were then established via selection with puromycin-containing medium. circPRKCI expression was tested by qPCR (a); Cell growth (b), viability (c), proliferation (d–f), migration (g), and apoptosis (h, i) were tested by appropriate assays mentioned in the text. For all the functional assays, the exact same amount of viable cells were plated into each well/dish initially (at 0 h) (Same for all Figures). “Pare” stands for the parental control cells. Error bars stand for mean ± standard deviation (SD, n = 5). *P < 0.05 vs. “Pare” cells. Experiments in this figure were repeated four times, and similar results were obtained. Bar = 100 μm (e, g)
