Fig. 5: circPRKCI silencing inhibits subcutaneous A172 glioma growth in SCID mice.
From: Circular RNA PRKCI promotes glioma cell progression by inhibiting microRNA-545
Weekly tumor growth curve of subcutaneous A172 gliomas with circPRKCI shRNA (“Seq-1/Seq-2”) or scramble non-sense control shRNA (“sh-c”) was shown (a); Estimated daily tumor growth was calculated using the described formula (b); At D35, tumors of all three groups were isolated and weighted (c); Mice body weights were recorded (d). At D7 and D14, one tumor of each group was isolated (total six tumors). Each tumor was randomly cut into five small pieces (n = 5), individually dissolved into the lysis buffer. Expression of circPRKCI (e), miR-545 (f), and listed proteins (g) in each piece was tested separately. Results were integrated and statistics analyses were performed (e, f). Representative blotting data were shown (g). Error bars stand for mean ± standard deviation (SD). *P < 0.05 vs. “sh-c” tumors
