Fig. 4: miR-30c enhanced DNA damage and genome instability following ADR treatment. | Cell Death & Disease

Fig. 4: miR-30c enhanced DNA damage and genome instability following ADR treatment.

From: Intrinsic adriamycin resistance in p53-mutated breast cancer is related to the miR-30c/FANCF/REV1-mediated DNA damage response

Fig. 4

a Genomic instability (mutation counts) was detected in TCGA BrCa patients (p < 0.05, n = 352). b, c MCF-7 and MDA-MB-231 cells were transfected with a negative control (NC) mimic (20 nM), miR-30c mimic (20 nM), ADR (0.5 µM) or miR-30c + ADR for 48 h. Chromosomes were then subjected to cytogenetic analysis. A total of 50 cells from each sample was examined and scored. Arrowheads indicate chromosome breaks. Magnification, ×400. For each sample, 50 comet tails were analyzed. *p < 0.05 vs. NC group, #p < 0.05 vs. ADR group. d Representative immunofluorescent images of γ-H2AX staining in MCF-7 and MDA-MB-231 cells treated with a negative control (NC) mimic (20 nM), miR-30c (20 nM), ADR(0.5 µM), or miR-30c + ADR for 48 h. Nuclei were counterstained with DAPI. For each group, 50 nuclei were analyzed. Magnification, ×200. Quantification results are shown on the right. *p < 0.05 vs. NC group, ##p < 0.01 vs. ADR group. Data represent the mean ± SD (n = 3, each group)

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