Fig. 8: Low testosterone levels inhibit TRPM8 activity by promoting accumulation of the TRPM8-AR complex in lipid rafts.
From: TRPM8-androgen receptor association within lipid rafts promotes prostate cancer cell migration
Top panel: in the absence of testosterone, the interaction between TRPM8 and AR takes place in the non-lipid raft domains (non cholesterol-rich domains) of the plasma membrane and does not affect TRPM8 activity and PCa cell migration. Middle panel:exposure of the PCa cells to low testosterone concentration (TST, 10 nM) promotes accumulation of TRPM8-AR complex in lipid rafts, where activated AR inhibits TRPM8 resulting in an increase of PCa cell migration. Bottom panel: exposure of the PCa cells to high testosterone concentration (TST, 100 nM) promotes AR internalization and TRPM8-AR complex dissociation. AR internalization induce a non-lipid rafts membrane localization of TRPM8 that does not affect TRPM8 activity and PCa cell migration
