Fig. 6: Notch signaling activation is crucial for VEGF165-mediated anti-fibrotic differentiation of stromal cells. | Cell Death & Disease

Fig. 6: Notch signaling activation is crucial for VEGF165-mediated anti-fibrotic differentiation of stromal cells.

From: Vascular endothelial growth factor 165 inhibits pro-fibrotic differentiation of stromal cells via the DLL4/Notch4/smad7 pathway

Fig. 6

a Endometrial stromal cells were stimulated with 10 ng/mL TGFβ1 for 48 h. Then these cells were treated with 10 μM Cyc for 24 h and/or cocultured with 10 ng/mL VEGF165 for another 12 or 24 h. The mRNA or protein levels of collagen 1, α-SMA and smad7 were examined. N=3, *P < 0.05. Analysis of mRNA levels was performed using two-way ANOVA and SNK-q. Analysis of protein levels was performed using one-way ANOVA and SNK-q. b Endometrial stromal cells were stimulated with 10 ng/mL TGFβ1 for 48 h. Then these cells were treated with 25 μM KYA1797K for 24 h and/or cocultured with 10 ng/mL VEGF165 for another 12 or 24 h. The mRNA or protein levels of collagen 1, α-SMA and smad7 were examined. N=3, *P < 0.05. Two-way ANOVA and SNK-q. c Endometrial stromal cells were stimulated with 10 ng/mL TGFβ1 for 48 h. Then these cells were treated with 30 μM DAPT for 24 h and/or cocultured with 10 ng/mL VEGF165 for another 12 or 24 h. The mRNA or protein levels of collagen 1, α-SMA and smad7 were examined. N=3, *P < 0.05. Analysis of mRNA levels was performed using two-way ANOVA and SNK-q. Analysis of protein levels was performed using one-way ANOVA and SNK-q. d-e Endometrial stromal cells stimulated with 10 ng/mL TGFβ1 for 48 h were treated with 10 ng/mL VEGF165 for another 24 h. The mRNA or protein levels of DLL4, Notch1 and Notch4 were examined. N=3, *P < 0.05. One-way ANOVA and SNK-q. T TGFβ1, VEGF VEGF165, Cyc cyclopamine

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