Fig. 6: The pancreatic cancer-associated SPOP mutation p.Q360* weakens the protein’s tumor suppression ability.
From: SPOP suppresses pancreatic cancer progression by promoting the degradation of NANOG
a Graphical representation of mutations of SPOP in pancreatic cancer. One “stop” mutation in SPOP was identified in the Genomic Data Commons (GDC) data sets (https://portal.gdc.cancer.gov/). Multispecies conservation of the mutated sites is shown below the mutation. NLS nuclear localization sequence. b Dynamic monitoring of the proliferation of SW1990 cells after transfection with Flag-SPOP-WT or Flag-SPOP-Q360* plasmids as indicated using the iCELLigence RTCA analyzer. ***P < 0.001. c Cell cycle analysis of SW1990 cells transfected with indicated plasmids using flow cytometry. Quantification of cell percentage is presented on the right panel. **P < 0.01. d Transwell assays were used to detect the migration and invasion abilities after transfection with Flag-SPOP-WT or Flag-SPOP-Q360* plasmids as indicated in SW1990 cells. Scale bar = 100 μm. Quantification of the stained colonies is shown in the below panel. Data are the average of three experiments (mean ± SD). *P < 0.05; **P < 0.01; ***P < 0.001. e Western blot analysis of WCL derived from SW1990 cells transfected with indicated plasmids
