Fig. 1: Identification of KEAP1 as a miR-421 target gene. | Cell Death & Disease

Fig. 1: Identification of KEAP1 as a miR-421 target gene.

From: MicroRNA-421 confers paclitaxel resistance by binding to the KEAP1 3′UTR and predicts poor survival in non-small cell lung cancer

Fig. 1

a Luciferase constructs containing the KEAP1 3′UTR. b Screening of miRNAs targeting KEAP1 by luciferase reporter assays. c Models of luciferase constructs containing the WT 3′UTR of KEAP1 and del6 (D6) 3′UTR of KEAP1. d HEK293 cells were co-transfected with NC and miR-421 with the WT or Mut 3′UTR. Luciferase activity was assayed 24 h after transient co-transfection. Renilla luciferase activity was measured and normalized to firefly luciferase activity. *P < 0.05 vs miR-421. e The protein level of KEAP1 in A549 cells was analysed by western blotting after transfection with miR-421. f LUADs from The Cancer Genome Atlas (TCGA). Expression values were retrieved from an RNAseq dataset (Illumina HiSeq)

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