Fig. 3: Assessment of palbociclib pharmacodynamics in vivo.

a Representative immunoblots of Ser780 phospho-Rb, total Rb, and actin for tumor cell lysates obtained from xenografts of parental cell-line control (gray) and 48 h of palbociclib treatment (red). b Corresponding cisplatin-resistant cell-line control (black) and 48 h of palbociclib treatment (blue). c Quantification by photo densitometry was performed on bands using Image Lab Software, and the ratio of phospho-Rb/total Rb was calculated. All quantification is presented as mean ± SD. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, by Student’s t-test)