Fig. 4: Parkin and BAG5 modulate Mcl-1 degradation following CCCP treatment.

a Representative western blot illustrating the changes in endogenous Mcl-1 in the GFP and GFP-BAG5 SH-SY5Y cell lines transfected with either pcDNA3.1 vector control or myc-Parkin, and treated with either 1 μm MG132, 50 μm CCCP, or a combination of the two. Representative image obtained from three independent experiments. b Quantification of Mcl-1 from western blots in three independent experiments in the absence of MG132. Data were obtained from three independent experiments and normalized to the GFP alone control condition in each experiment. c Quantification of Mcl-1 from western blots in independent experiments in the presence of MG132. Data were obtained from three independent experiments and normalized to the GFP alone control condition in each experiment. Statistical analysis in b and c was done using one-way ANOVA followed by Bonferroni post hoc testing (*p < 0.05, **p < 0.01, ***p < 0.001). Columns represent mean ± SEM.