Fig. 1: ZFAS1 knockdown inhibits cardiomyocyte apoptosis caused by myocardial infarction. | Cell Death & Disease

Fig. 1: ZFAS1 knockdown inhibits cardiomyocyte apoptosis caused by myocardial infarction.

From: lncRNA-ZFAS1 induces mitochondria-mediated apoptosis by causing cytosolic Ca2+ overload in myocardial infarction mice model

Fig. 1

a Effects of ZFAS1 knockdown on the mitochondrion in the myocardium of MI mice, observed by electron microscope. Yellow arrows pointing to the mitochondrion. Images are presented with a magnification of ×10,000 for the top and ×40,000 for the down panel. It was repeated for three times with similar results. b MTT assay showing that ZFAS1 knockdown increased the cell viability of hypoxia-treated cells. *P < 0.05 vs. Hypoxia + siNC, n = 13. c, d LIVE/DEAD Viability/Cytotoxicity Kit stains were used to detect the effects of ZFAS1 on cardiomyocytes. Cells with compromised membranes exhibit red-fluorescence, while those with intact cell membranes show bright green-fluorescence. *P < 0.05 vs. control group, n = 5; #P < 0.05 vs. hypoxia group, n = 5. Magnification, ×200. e Effects of ZFAS1 knockdown on the mitochondrion in hypoxia-treated cardiomyocytes, visualized by electron microscope (magnification, ×10,000 for the top and ×40,000 for the down panel). Similar results were consistently observed in another two batches of cells. f ZFAS1 knockdown improved the mitochondrial membrane potential, as observed by JC-1 staining. The red-fluorescent aggregates (J-aggregates) represent high membrane potential; green fluorescence (JC-1 monomers) represents dissipated mitochondrial membrane potential. Magnification, ×1200. Similar results were consistently observed in another two batches of cells. g, h The influence on apoptosis with ZFAS1 knockdown was examined via TUNEL assay. Blue, DAPI staining for nucleus; green, TUNEL-positive staining for apoptotic cells. **P < 0.01 vs. control group, #P < 0.05 vs. hypoxia group, the results are expressed as the means ± SEM of four independent experiments. Magnification, ×200. i, j Cells were harvested and processed for apoptosis assay using the Annexin V-FITC Apoptosis Detection Kit by flow cytometry. *P < 0.05 vs. control group, #P < 0.05 vs. hypoxia group, the results are expressed as the means ± SEM of four independent experiments.

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