Fig. 6: CDK8 and LRP6 were targeted by miR-26a/b-5p and were under regulation of DLGAP1-AS1. | Cell Death & Disease

Fig. 6: CDK8 and LRP6 were targeted by miR-26a/b-5p and were under regulation of DLGAP1-AS1.

From: Long non-coding RNA DLGAP1-AS1 facilitates tumorigenesis and epithelial–mesenchymal transition in hepatocellular carcinoma via the feedback loop of miR-26a/b-5p/IL-6/JAK2/STAT3 and Wnt/β-catenin pathway

Fig. 6

a The binding sites for miR-26a-5p (left) and miR-26b-5p (right) within the 3′-UTR sequences of CDK8 (top) and LRP6 (bottom) were exhibited through starBase prediction. b qRT-PCR evaluated CDK8 and LRP6 mRNA levels in HCC cell lines and in normal cells. c WB analysis evaluated CDK8 and LRP6 protein levels in HCC cell lines and in normal cells. d, e The effects of DLGAP1-AS1 knockdown in Hep G2 cells and DLGAP1-AS1 overexpression in SNU-387 cells on CDK8 or LRP6 expression were exhibited using qRT-PCR and WB. f RIP assay was performed using the Ago2 antibody to demonstrate the enrichment of DLGAP1-AS1, miR-26a/b-5p and mRNAs of CDK8 and LRP6 in HCC cells. g RNA pull-down assay was performed to detect the binding ability of CDK8 or LRP6 mRNA with miR-26a/b-5p. h Luciferase reporter assay elucidated the interaction between CDK8 or LRP6 mRNA and miR-26a/b-5p, and the competing effect of DLGAP1-AS1 to interact with miR-26a/b-5p. All data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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