Fig. 2: Upregulation of UBE2O promoted BC cell proliferation. | Cell Death & Disease

Fig. 2: Upregulation of UBE2O promoted BC cell proliferation.

From: UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop

Fig. 2

a Protein expression of UBE2O in a panel of BC cells and nontransformed MCF-10A cells was detected by western blotting. b CCK-8 and c colony formation assays showed that knocking down UBE2O inhibited the proliferation ability of MDA-MB-231 cells. Conversely, overexpression of UBE2O in MCF-7 cells promoted cell proliferation. d The expression status of UBE2O and Ki-67 was examined by IHC (upper: magnification × 100, Scale bar, 100 μm; lower: magnification × 400, Scale bar, 20 μm), and their relevance was analysed by a chi-square test (Ki-67 < 20% was regarded as low expression). e–g Mouse xenograft models were used to investigate the tumourigenesis of MDA-MB-231sh-UBE2O#1 cells in vivo (upper: magnification × 100, Scale bar, 100 μm; lower: magnification × 400, Scale bar, 20 μm), f the volumes of tumours established in mice in the MDA-MB-231sh-NC/MDA-MB-231sh-UBE2O#1 groups were recorded, and g the tumour-free survival of the two groups was analysed. The data are shown as the mean ± s.d. Student’s t test was used for statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001. The data represent at least three independent experiments.

Back to article page