Fig. 3: Upregulation of UBE2O promoted BC cell migration and invasion. | Cell Death & Disease

Fig. 3: Upregulation of UBE2O promoted BC cell migration and invasion.

From: UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop

Fig. 3

a Wound healing assays were performed to detect the effect of UBE2O expression on migration in the indicated cells (Scale bar, 200 μm). b Invasion abilities were examined by Matrigel invasion assays after UBE2O expression levels were changed in the indicated cells (Scale bar, 200 μm). c Western blot assays revealed that the epithelial markers (CDH1) were increased, and the mesenchymal markers (CDH2, vimentin and slug) were reduced after inhibiting UBE2O in MDA-MB-231 cells; the opposite results occurred in MCF-7OE-UBE2O cells in comparison with control cells. d IF staining assays were used to explore EMT markers in MDA-MB-231sh-NC/MDA-MB-231sh-UBE2O#1 and MCF-7NC/MCF-7OE-UBE2O cells (Scale bar, 50 μm). e Lungs excised from MDA-MB-231sh-NC/MDA-MB-231sh-UBE2O#1 mice were photographed, and lung metastases were subjected to HE staining and analysed (Scale bar, 100 μm). The data are shown as the mean ± s.d. Student’s t test was used for statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001. The data represent at least three independent experiments.

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