Fig. 8: MYC transcriptionally promoted UBE2O expression, and a positive feedback loop existed in BC cells. | Cell Death & Disease

Fig. 8: MYC transcriptionally promoted UBE2O expression, and a positive feedback loop existed in BC cells.

From: UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop

Fig. 8

a Two putative MYC-binding sites in the UBE2O promoter region were forecasted by the JASPAR database. b, c siRNAs targeting MYC were transfected into MDA-MB-231 and MCF-7 cells. Expression changes in UBE2O were detected by qRT-PCR and western blot assays. d Wild-type or mutant UBE2O promoter reporter constructs targeting the two putative binding sites of MYC were separately transfected into the 293 T cell line. Various amounts of MYC plasmids were also transfected, and luciferase assays were performed. e ChIP assays were performed in the indicated BC cells with IgG or MYC antibodies. qRT-PCR was used to detect the fragments of the UBE2O promoter region. f Structure chart showing that the UBE2O/AMPKα2/mTORC1/MYC axis constituted a positive feedback loop in BC cells. The data are shown as the mean ± s.d. Student’s t test was used for statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001. The data represent at least three independent experiments.

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