Fig. 1: H. pylori-infected gastric epithelial cells are resistant to Raptinal-induced apoptosis.
From: H. pylori infection confers resistance to apoptosis via Brd4-dependent BIRC3 eRNA synthesis
a Indicated cell lines were treated with 10 μM of Raptinal or Cisplatin for 2 or 24 h, respectively. Cell lysates were analyzed by immunoblotting for indicated proteins. b Indicated gastric cancer cell lines were treated with 10 µM of Raptinal for 72 h, and cell proliferation was measured by A490 nm using the CellTiter 96RAQueous One Solution cell proliferation assay (MTS) (Promega). c AGS cells infected with H. pylori G27 for 1 h followed by treatment with indicated concentration of Raptinal for 2 h. Cell lysates were analyzed by immunoblotting for indicated proteins. d AGS cells infected with H. pylori G27 for 1 h followed by treatment with Raptinal (10 µM) for 2 h. Apoptosis was assessed by flow cytometry using Annexin V-FITC/PI double staining assay. e The percentage of apoptotic cells from (d). All data are shown as the mean ± SD from three independent experiments. *p < 0.05, ***p < 0.001, n.s.: not significant.
