Fig. 4: PIK3R3 combines p53 and enhances its transcriptional activity.
From: PIK3R3 inhibits cell senescence through p53/p21 signaling
a Immunoprecipitation assays were performed for LoVo cells with anti-PIK3R3 or anti-p53 antibodies. b Immunoprecipitation assays with antibodies against Flag or His in LoVo tranfected with Flag-PIK3R3 or His-p53. c, d ChIP assays of p53 binding to the CDKN1A promoter region in LoVo transfected with siPIK3R3#1. e ChIP assays of p53 binding to the CDKN1A promoter region in LoVo transfected with PIK3R3-expressing plasmid. f WB of PIK3R3, p53, and p21 in LoVo and SW48 cells transfected with siPIK3R3#1 or/and sip53. g WB of PIK3R3, p53, and p21 in LoVo and SW48 cells transfected with PIK3R3 plasmid or/and His-p53 plamid. Every assay was repeated three times, and these numeral results were displayed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, t-test.
