Fig. 1: UBAC2 is overexpressed in BC tissues and cell lines.

a The results from qRT-PCR analysis showed that the mRNA expression levels of UBAC2 were significantly upregulated in BC tissues compared with their adjacent normal tissues. β-actin was used as loading control. b The protein expression levels of UBAC2 in 12 samples were determined by immunohistochemistry (IHC) staining and analyzed by calculating the integrated optical density per stained area (IOD/area). The tumor sample shown here is stage T3 with high grade. c, d The mRNA (c) and protein (d) levels of UBAC2 in SV-HUC-1, RT4, EJ, UMUC3, T24, and T24T cells were validated by qRT-PCR and western blotting. β-actin was used as loading control. e Kaplan–Meier survival plots of 48 bladder cancer cases from our hospital show the survival probability of patients with high or low expression of UBAC2 (p = 0.030, log-rank test). f Kaplan–Meier survival plots of 406 bladder cancer cases from TCGA database show the survival probability of patients with high or low expression of UBAC2 (n = 406, p = 0.035, log-rank test). g Immunofluorescence (IF) showed that UBAC2 protein stained green was localized in cytoplasm of BC cells. Nuclei were stained blue with DAPI (scale bar, 10 μm). Data are presented as mean ± SEM from three independent replicates. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t test).