Fig. 3: Knockdown of UBAC2 increases the expression of p27.
From: UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis
a The expression levels of cell cycle related proteins (cyclin D1, cyclin E, CDK2, CDK4, CDK6, p27, and p21) were analyzed by western blotting after transfection with shUBAC2 or shNC in EJ and UMUC3 cell lines. β-actin was used as loading control. b The results from qRT-PCR showed that knockdown of UBAC2 significantly promoted the mRNA expression of p27 in EJ and UMUC3 cell lines. β-actin was used as loading control. c The protein expression level of UBAC2 and p27 in xenograft tumors was detected by immunohistochemistry (IHC) staining and analyzed by calculating the integrated optical density per stained area (IOD/area). d The mRNA expression level of UBAC2 and p27 in xenograft tumors from nude mice was determined by qRT-PCR. e, f Cell cycle distributions in shNC group, shUBAC2-3 group, shP27 group and shUBAC2-3 + shP27 group were presented by flow cytometry. Data are presented as means ± SEM from three independent replicates or as means ± SEM of five mice from each group. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test).
