Fig. 6: Overexpression of miR-182-5p partly reversed the effects of shUBAC2.
From: UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis

a, b Western blotting (a) and qRT-PCR (b) analysis of the expression levels of p27 in the EJ and UMUC3 after co-transfection with shUBAC2-3 and miR-182-5p mimics. β-actin was used as loading control. c, d Cell cycle distributions in shNC + mimic NC group, shUBAC2-3 + mimic NC group, shNC + miR-182-5p group and shUBAC2-3 + miR-182-5p group were presented by flow cytometry. Data are presented as means ± SEM from three independent replicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test).