Fig. 1: miR-196b-5p plays an oncogenic function in lung cancer cells and is negatively correlated with FAS in non-small cell lung cancer (NSCLC). | Cell Death & Disease

Fig. 1: miR-196b-5p plays an oncogenic function in lung cancer cells and is negatively correlated with FAS in non-small cell lung cancer (NSCLC).

From: miR-196b-5p-mediated downregulation of FAS promotes NSCLC progression by activating IL6-STAT3 signaling

Fig. 1

a miR-196b-5p expression data were obtained from the TCGA miR-seq dataset. Of evaluable 666 NSCLC patients, 86 patients had matched normal adjacent tissues. b qRT-PCR examines miR-196b-5p expression in miR-196b-5p overexpressing H460 cells and control cells. Results are represented as means ± SD (n = 3). c Cell proliferation assay for miR-196b-5p overexpressing H460 cells and control cells. Results are represented as means ± SD (n = 3). d Colony-formation assay for miR-196b-5p overexpressing H460 cells and control cells. Colony-forming areas were measured by Image J software, and relative colony-forming areas were calculated by comparing with corresponding controls. Results are represented as means ± SD (n = 3). e, f FAS expression from TCGA RNA-seq data and miR-196b-5p expression from miR-seq data were used to examine the correlation between miR-196b-5p and FAS expressions in lung ADC dataset (n = 469) (e) and lung squamous cell carcinoma (SCC) dataset (n = 378) (f). ***P < 0.001, ****P < 0.0001.

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