Fig. 3: FAS knockdown promotes lung cancer cell proliferation and cell cycle. | Cell Death & Disease

Fig. 3: FAS knockdown promotes lung cancer cell proliferation and cell cycle.

From: miR-196b-5p-mediated downregulation of FAS promotes NSCLC progression by activating IL6-STAT3 signaling

Fig. 3

a qRT-PCR measure FAS expression in FAS knockdown A549 and H292 lung cancer cells. The expression was normalized by GAPDH. Results are represented as means ± SD (n = 3). b Western blot analyses of FAS protein in FAS knockdown A549 and H292 lung cancer cells. The bands were quantified using Image J software, and relative values were obtained by normalizing to the value of each corresponding GAPDH. c Cell proliferation assay for A549 and H292 cells after transfection with siFAS for 4 days. Results are represented as means ± SD (n = 3). d The percentage of cell cycle G1 phase cells in FAS knockdown A549 lung cancer cells and control cells. Results are represented as means ± SD (n = 3). e Western blot analyses for cell cycle G1 phase-related proteins in FAS knockdown A549 lung cancer cells and control cells. The bands were quantified using Image J software, and relative values were obtained by normalizing to the value of each corresponding Vinculin. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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