Fig. 6: FAS knockdown-mediated p65 activation promotes IL6 expression. | Cell Death & Disease

Fig. 6: FAS knockdown-mediated p65 activation promotes IL6 expression.

From: miR-196b-5p-mediated downregulation of FAS promotes NSCLC progression by activating IL6-STAT3 signaling

Fig. 6

a qRT-PCR measure IL6 expression in p65 overexpressing A549 cells and control cells. The expression was normalized by GAPDH. Results are represented as means ± SD (n = 3). b ELISA measures secreted IL6 protein levels in conditioned media from p65 overexpressing A549 cells and control cells. c Western blot analysis for p-STAT3 (Y705) expression in A549 cells treated with conditioned mediums from p65 overexpressing lung cancer cells or control cells. The p-STAT3 bands were quantified using Image J software, and relative values were obtained by normalizing to the value of the corresponding STAT3. d Cell proliferation assay of A549 cells treated with conditioned mediums from p65 overexpressing lung cancer cells or control cells. Results are represented as means ± SD (n = 3). e Western blot analyses for p-STAT3 (Y705), STAT3, p-Jak2, and Jak2 in miR-196b-5p overexpressing lung cancer cells and control cells. f Western blot analyses for p65 protein in miR-196b-5p overexpressing A549 cells and control cells. Both nuclear and cytosol proteins were extracted and subjected to western blot analysis. **P < 0.01, ****P < 0.0001.

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