Fig. 3: Bip knockdown enhances the activation of IR-induced specific UPR branch pathways.

GSCs transfected or untransfected with siRNA-Bip were treated with IR 10 Gy in the presence or absence of UPR pathway inhibitors, then UPR proteins and DAMPs were detected at 24 h after irradiation. a The changes of PERK pathway proteins and DAMPs were analyzed with or without pretreatment with 2 μM GSK2606414, a specific PERK pathway inhibitor, for 1 h before irradiation. b The expressions of IRE1-α pathway proteins and DAMPs were measured in presence or absence of 10 μM 4u8c, a specific IRE1-α pathway inhibitor, for 1 h before irradiation. c Protein level of ATF6 was determined by immunoblot. d The mechanism of IR-mediated decreased ICD in GSCs. *P < 0.05, **P < 0.01 vs control, ^#P < 0.05, ^##P < 0.01 vs IR.